Robert Hill, APRN
Staff Affiliate - YNHHAbout
Research
Publications
2026
Myelin sheaths persist for weeks following axon degeneration
Doty M, Tuckerman E, Piedra E, Hill R. Myelin sheaths persist for weeks following axon degeneration. Science Advances 2026, 12: eaeb2628. PMID: 41779849, PMCID: PMC12959408, DOI: 10.1126/sciadv.aeb2628.Peer-Reviewed Original Research
2025
TREM2 and APOE are dispensable for microglial clearance of dying neurons revealed by in vivo imaging
Rai A, Damisah E, Hill R, Tong L, Grutzendler J. TREM2 and APOE are dispensable for microglial clearance of dying neurons revealed by in vivo imaging. IScience 2025, 29: 114559. PMID: 41660266, PMCID: PMC12877822, DOI: 10.1016/j.isci.2025.114559.Peer-Reviewed Original ResearchAbsence of TREM2Dying CellsImpact of TREM2Programmed Cell DeathAlzheimer's diseaseRisk genesMicroglial clearanceAD riskProtein aggregationCell deathTREM2Cell bodiesAmyloid depositsMicroglial cell bodiesDying neuronsMechanism IndependentApoCellular debrisRemoval of dying neuronsCellsDeletionGenesAdult miceAmyloidProteinMitochondria are absent from microglial processes performing surveillance, chemotaxis, and phagocytic engulfment
Pietramale A, Bame X, Doty M, Schwarz K, Hill R. Mitochondria are absent from microglial processes performing surveillance, chemotaxis, and phagocytic engulfment. Nature Communications 2025, 16: 11104. PMID: 41387721, PMCID: PMC12700918, DOI: 10.1038/s41467-025-66708-6.Peer-Reviewed Original ResearchConceptsSubcellular heterogeneityPhagocytic engulfmentProgrammed Cell DeathMitochondrial partitioningMitochondrial motilityMitochondrial organizationMicroglial processesMitochondrial distributionMultiple mitochondriaMitochondriaTissue homeostasisCell deathMicroglial metabolismMitochondrial contentCell processesUltrastructural analysisHuman tissuesDetection of tissue damageEngulfmentIntravital imagingMetabolic componentsMicrotubulesMicrogliaMotilityHexokinaseLabel-free and fluorescence imaging of oligodendrocytes and myelin
Kamen Y, Doty M, Ciolkowski M, Bame X, Pietramale A, Schain A, Grutzendler J, Hill R. Label-free and fluorescence imaging of oligodendrocytes and myelin. Npj Imaging 2025, 3: 33. PMID: 40670677, PMCID: PMC12267544, DOI: 10.1038/s44303-025-00098-8.Peer-Reviewed Original ResearchTransient Upregulation of Procaspase-3 during Oligodendrocyte Fate Decisions
Kamen Y, Chapman T, Piedra E, Ciolkowski M, Hill R. Transient Upregulation of Procaspase-3 during Oligodendrocyte Fate Decisions. Journal Of Neuroscience 2025, 45: e2066242025. PMID: 39837665, PMCID: PMC11924999, DOI: 10.1523/jneurosci.2066-24.2025.Peer-Reviewed Original ResearchProcaspase-3Fate decisionsOligodendrocyte precursor cellsCell death mechanismsNeurodegenerative conditionsOligodendrocyte differentiationSurvival decisionsCellular checkpointsDeath mechanismsMolecular markersPrecursor cellsOligodendrocyte markersPromote oligodendrocyte differentiationPharmacological inhibitionDifferentiation stageTransient upregulationDifferentiationZymogenCellsOligodendrocyte densityMorphological stateMale miceOligodendrocyte deathMyelin dysfunctionMarkers
2024
Mitochondrial network reorganization and transient expansion during oligodendrocyte generation
Bame X, Hill R. Mitochondrial network reorganization and transient expansion during oligodendrocyte generation. Nature Communications 2024, 15: 6979. PMID: 39143079, PMCID: PMC11324877, DOI: 10.1038/s41467-024-51016-2.Peer-Reviewed Original ResearchConceptsDecreased mitochondrial sizeLoss of mitochondriaMitochondrial motilityMitochondrial dynamicsCellular checkpointsMitochondrial distributionMitochondrial sizeMitochondrial contentOligodendrocyte precursor cellsSubcellular partitioningDistal processesMotilityOligodendrocyte generationOligodendrocyte processesLocal microenvironmentPrecursor cellsExtensive expansionMitochondriaOligodendrocyte lineageTransient expansionLineagesOligodendrocytesAging BrainMyelinating oligodendrocytesMorphometricsMicroglial phagocytosis of single dying oligodendrocytes is mediated by CX3CR1 but not MERTK
Olveda G, Barasa M, Hill R. Microglial phagocytosis of single dying oligodendrocytes is mediated by CX3CR1 but not MERTK. Cell Reports 2024, 43: 114385. PMID: 38935500, PMCID: PMC11304498, DOI: 10.1016/j.celrep.2024.114385.Peer-Reviewed Original ResearchOligodendrocyte Maturation Alters the Cell Death Mechanisms That Cause Demyelination
Chapman T, Kamen Y, Piedra E, Hill R. Oligodendrocyte Maturation Alters the Cell Death Mechanisms That Cause Demyelination. Journal Of Neuroscience 2024, 44: e1794232024. PMID: 38395617, PMCID: PMC10977033, DOI: 10.1523/jneurosci.1794-23.2024.Peer-Reviewed Original ResearchPoly-ADP-riboseCell deathSubcellular localizationCaspase-3-independent mechanismCombination of intravital imagingCaspase-3-dependent mechanismDNA damageMechanism of cell deathProgrammed Cell DeathPrevent cell deathCell death mechanismsExecutioner caspasesDelayed cell deathOligodendrocyte maturationCaspase-3Mature oligodendrocytesOligodendrocyte deathDeath mechanismsHuman diseasesHallmarks of diseaseMolecular mechanismsIndividual cellsMechanisms of oligodendrocyte deathIntravital imagingSingle-cell ablation
2023
Features, Fates, and Functions of Oligodendrocyte Precursor Cells.
Hill R, Nishiyama A, Hughes E. Features, Fates, and Functions of Oligodendrocyte Precursor Cells. Cold Spring Harbor Perspectives In Biology 2023, 16: a041425. PMID: 38052500, PMCID: PMC10910408, DOI: 10.1101/cshperspect.a041425.Peer-Reviewed Original Research
2014
Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination
Hill R, Medved J, Patel K, Nishiyama A. Organotypic Slice Cultures to Study Oligodendrocyte Dynamics and Myelination. Journal Of Visualized Experiments 2014 DOI: 10.3791/51835-v.Peer-Reviewed Original Research