Ying Wu, PhD
Associate Research ScientistAbout
Research
Publications
2026
Anti-CD3 mAb treatment reshapes infiltrating T and β cells in the islets in autoimmune diabetes
Wu Y, Spurrell M, Lledó-Delgado A, Deng S, Wang D, Liu Y, Barkestani M, Perdigoto A, Herold K. Anti-CD3 mAb treatment reshapes infiltrating T and β cells in the islets in autoimmune diabetes. JCI Insight 2026, 11: e192755. PMID: 41574610, PMCID: PMC12892913, DOI: 10.1172/jci.insight.192755.Peer-Reviewed Original ResearchConceptsAnti-CD3 mAb treatmentT cellsMAb treatmentAutoimmune diabetesB cellsCD8+ T cellsIslet-infiltrating T cellsAnti-CD3 monoclonal antibodyT-cell receptor sequencingPrediabetic NOD miceAutoreactive T cellsPancreatic lymph nodesImmune-mediated destructionCourse of treatmentType 1 diabetesInfiltrating TSingle-cell RNAIslet infiltrationMAb-treatedNOD miceLymph nodesEffector functionsOperational toleranceMonoclonal antibodiesProlonged effect
2022
RAGE antagonism with azeliragon improves xenograft rejection by T cells in humanized mice.
Joshi AA, Wu Y, Deng S, Preston-Hurlburt P, Forbes JM, Herold KC. RAGE antagonism with azeliragon improves xenograft rejection by T cells in humanized mice. Clinical Immunology 2022, 245: 109165. PMID: 36257528, DOI: 10.1016/j.clim.2022.109165.Peer-Reviewed Original ResearchConceptsXenograft rejectionIL-17AHumanized miceIL-1βT cellsImmune responseRAGE antagonistsAdaptive human immune responsesPD-1 expressionSkin graft rejectionHuman immune cell responsesImmune cell responsesHuman immune responseHuman immune cellsInnate immune responseAdvanced glycation endproductsInhibition of pathwaysSmall molecule antagonistsMultiple inflammatory processesAZ therapyRAGE antagonismGraft rejectionIL-23Serum levelsMedian time
2021
Development of a fully canine anti-canine CTLA4 monoclonal antibody for comparative translational research in dogs with spontaneous tumors
Mason N, Chester N, Xiong A, Rotolo A, Wu Y, Yoshimoto S, Glassman P, Gulendran G, Siegel D. Development of a fully canine anti-canine CTLA4 monoclonal antibody for comparative translational research in dogs with spontaneous tumors. MAbs 2021, 13: 2004638. PMID: 34856888, PMCID: PMC8726733, DOI: 10.1080/19420862.2021.2004638.Peer-Reviewed Original ResearchConceptsImmune checkpoint inhibitorsSpontaneous tumorsNon-small cell lung carcinomaClinical response to treatmentBinding to CD80/CD86Immune-responsive cancersAnti-tumor immunityBiomarkers of responseResponse to chemotherapyPromote T cell proliferationCell lung carcinomaRenal cell carcinomaT cell proliferationIn vivo mouse studiesTreatment of patientsShort-term adverse effectsResponse to treatmentPredictors of outcomeIsotype control IgGCTLA4 antibodyICI combinationsCheckpoint blockadeCheckpoint inhibitionCheckpoint inhibitorsImmune signatures
2020
Immunoregulatory Cells in Myasthenia Gravis
Wu Y, Luo J, Garden O. Immunoregulatory Cells in Myasthenia Gravis. Frontiers In Neurology 2020, 11: 593431. PMID: 33384654, PMCID: PMC7769807, DOI: 10.3389/fneur.2020.593431.Peer-Reviewed Original ResearchMyeloid-derived suppressor cellsExperimental autoimmune MGMG patientsImmunoregulatory cellsCD4+ Foxp3+ regulatory T cellsCD4+ Foxp3+ TregsFoxp3+ regulatory T cellsIL-10-producing B cellsPopulations of immunoregulatory cellsFollicular T helper cellsBiomarkers of disease activityFoxP3+ TregsRegulatory T cellsImmunoregulatory cell populationsT helper cellsB-cell mediated autoimmune diseasesPathogenesis of MGHuman peripheral bloodContext of MGFollicular TregsAdoptive transferSuppressor cellsAutoimmune MGPeripheral bloodT cellsMyeloid-derived suppressor cell and regulatory T cell frequencies in canine myasthenia gravis: A pilot study
Wu Y, Chang Y, Lawson B, Galban E, Mittelman N, Benedicenti L, Petesch S, Carroll A, Punt J, Luo J, Garden O. Myeloid-derived suppressor cell and regulatory T cell frequencies in canine myasthenia gravis: A pilot study. The Veterinary Journal 2020, 267: 105581. PMID: 33375962, PMCID: PMC7780263, DOI: 10.1016/j.tvjl.2020.105581.Peer-Reviewed Original ResearchConceptsMyeloid-derived suppressor cellsFrequency of myeloid-derived suppressor cellsPeripheral blood of dogsBlood of dogsMyasthenia gravisSeronegative dogsSuppressor cellsHealthy dogsRegulatory T cell frequencyB-cell-mediated autoimmune diseasesClinical course of diseaseAnti-AChR antibody titerT cell frequenciesRegulatory T cellsDogsCourse of diseaseCanine myasthenia gravisAntibody titersGeneralized MGImmunosuppressed populationClinical coursePeripheral bloodT cellsAnti-AChRProspective studyGene Expression Profiling of B Cell Lymphoma in Dogs Reveals Dichotomous Metabolic Signatures Distinguished by Oxidative Phosphorylation
Wu Y, Chang Y, Polton G, Stell A, Szladovits B, Macfarlane M, Peters L, Priestnall S, Bacon N, Kow K, Stewart S, Sharma E, Goulart M, Gribben J, Xia D, Garden O. Gene Expression Profiling of B Cell Lymphoma in Dogs Reveals Dichotomous Metabolic Signatures Distinguished by Oxidative Phosphorylation. Frontiers In Oncology 2020, 10: 307. PMID: 32211332, PMCID: PMC7069556, DOI: 10.3389/fonc.2020.00307.Peer-Reviewed Original ResearchCell of originGene expression profilesCanine non-Hodgkin's lymphomaNon-Hodgkin's lymphomaB-cell lymphomaDLBCL subtypesHuman DLBCLMolecular subtypesDLBCLMetabolic classificationOxidative phosphorylationTranscriptomic phenotypesMolecular heterogeneityLymphomaMolecular phenotypesSubtypesExpression profilesPilot studyPhenotype
2019
Phenotypic characterisation of regulatory T cells in dogs reveals signature transcripts conserved in humans and mice
Wu Y, Chang Y, Stell A, Priestnall S, Sharma E, Goulart M, Gribben J, Xia D, Garden O. Phenotypic characterisation of regulatory T cells in dogs reveals signature transcripts conserved in humans and mice. Scientific Reports 2019, 9: 13478. PMID: 31530890, PMCID: PMC6748983, DOI: 10.1038/s41598-019-50065-8.Peer-Reviewed Original ResearchConceptsRegulatory T cellsT cellsT cells isolated ex vivoSubsets of TregsTranscriptomic signaturesAvailability of mAbsHealthy dogsTreg functionMurine TregsVeterinary speciesTregsRegulatory phenotypeNeoplastic disordersSurface antigensImmune systemMonoclonal antibodiesDogsCross-species comparisonsMicePhenotypic and transcriptomic characterization of canine myeloid-derived suppressor cells
Goulart M, Hlavaty S, Chang Y, Polton G, Stell A, Perry J, Wu Y, Sharma E, Broxholme J, Lee A, Szladovits B, Turmaine M, Gribben J, Xia D, Garden O. Phenotypic and transcriptomic characterization of canine myeloid-derived suppressor cells. Scientific Reports 2019, 9: 3574. PMID: 30837603, PMCID: PMC6400936, DOI: 10.1038/s41598-019-40285-3.Peer-Reviewed Original ResearchConceptsMyeloid-derived suppressor cellsPMN-MDSCsM-MDSCsSuppressor cellsMyeloid-derived suppressor cell subsetsMyeloid derived suppressor cell functionPeripheral blood frequenciesPolymorphonuclear (PMN)-MDSCsMonocytic (M)-MDSCsPhenotypically distinct subsetsHuman tumor developmentCanine oncology patientsTumor progressionImmune evasionTumor developmentContext of cancerOncology patientsTranscriptomic signaturesGene expression patternsPathological statesCancerExpression patternsCellsMetastasisSubsets
2018
Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry
Pittaway R, Wu Y, Szladovits B, Suárez-Bonnet A, Scurrell E, Garden O, Polton G, Priestnall S. Diagnosis of anaplastic large-cell lymphoma in a dog using CD30 immunohistochemistry. Journal Of Veterinary Diagnostic Investigation 2018, 30: 455-458. PMID: 29455626, PMCID: PMC6505805, DOI: 10.1177/1040638718760965.Peer-Reviewed Original ResearchConceptsAnaplastic large-cell lymphomaLarge-cell lymphomaNull-cell lymphomasDiagnosis of anaplastic large-cell lymphomaCases of lymphomaT lymphocyte markersCD30 immunohistochemistryNull-cellPotential therapeutic targetClinical entityPrognostic informationT lymphocytesPoor prognosisLymphomaCD30Pleomorphic cellsNeoplastic cellsFlow cytometryTherapeutic targetImmunohistochemistryCellsDogsCD3CD79aUnique appearance
2017
Phenotypic heterogeneity of peripheral monocytes in healthy dogs
Gibbons N, Goulart M, Chang Y, Efstathiou K, Purcell R, Wu Y, Peters L, Turmaine M, Szladovits B, Garden O. Phenotypic heterogeneity of peripheral monocytes in healthy dogs. Veterinary Immunology And Immunopathology 2017, 190: 26-30. PMID: 28778319, DOI: 10.1016/j.vetimm.2017.06.007.Peer-Reviewed Original Research