Ruben De Man
About
Research
Publications
2026
Proximal Pulmonary Artery Stiffening as a Biomarker of Cardiopulmonary Aging
De Man R, Cai Z, Doddaballapur P, Guerrera N, Regan A, Lin L, Schwarz E, Justet A, Abu Hussein N, Di Palo J, Cavinato C, Raredon M, Heerdt P, Singh I, Yan X, Kang M, Bruns D, Lee P, Tellides G, Humphrey J, Kaminski N, Ramachandra A, Manning E. Proximal Pulmonary Artery Stiffening as a Biomarker of Cardiopulmonary Aging. Aging Cell 2026, 25: e70383. PMID: 41589414, PMCID: PMC12836046, DOI: 10.1111/acel.70383.Peer-Reviewed Original ResearchConceptsProximal pulmonary arteriesPulmonary arterySmooth muscle cellsPerivascular macrophagesMouse modelMuscle cellsRight ventricle functionMedial smooth muscle cellsPulmonary arterial stiffeningRight ventricleVentricle functionAssociated with senescenceECM turnoverLung functionArterial stiffeningArteryAdventitial fibroblastsMolecular targetsAge-relatedGeroscience hypothesisLungAgeIntercellular signalingMiceMacrophagesSingle-cell atlas of human lung aging identifies cell type dyssynchrony and increased transcriptional entropy
De Man R, McDonough J, Adams T, Nikola F, Rangel R, Anderson S, Manning E, Cala Garcia J, Moss B, Waich A, Poli F, Cardenas R, Coarfa C, Song Q, Bar-Joseph Z, Vanaudenaerde B, Wuyts W, Niklason L, Raredon M, Yan X, Rosas I, Kaminski N. Single-cell atlas of human lung aging identifies cell type dyssynchrony and increased transcriptional entropy. Nature Communications 2026, 17: 2095. PMID: 41571679, PMCID: PMC12953888, DOI: 10.1038/s41467-026-68810-9.Peer-Reviewed Original ResearchConceptsGenomic landscapeLung ageSingle-cell atlasAlveolar epithelialIndependent predictorsAnalysis of somatic mutationsSingle-cell dataSingle-cell RNA sequencingEndothelial cellsLoss of differentiationAlveolar epithelial cellsRNA sequencingTranscriptional changesCell-typeEndothelial cell typesSomatic mutationsMutational burdenAT2 cellsLung diseaseCell typesAge-related changesEpithelial cellsRisk factorsSenescence signatureEpithelial
2025
Understanding and Reducing Cardiopulmonary Sequelae Associated With Chronic Hypoxia
Manning E, Ramachandra A, Sharma P, Nikola F, De Man R, Doddaballapur P, Raredon M, Szafron J, Baernthaler T, Justet A, Akingbesote N, Perry R, Adams T, Cavinato C, Yan X, Tellides G, Kaminski N, Humphrey J. Understanding and Reducing Cardiopulmonary Sequelae Associated With Chronic Hypoxia. American Journal Of Respiratory And Critical Care Medicine 2025, 211: a3336-a3336. DOI: 10.1164/ajrccm.2025.211.abstracts.a3336.Peer-Reviewed Original ResearchSingle-cell Analysis Reveals Somatic Mutation Accumulation, Loss of Differentiation, and Increased Transcriptional Entropy in Aged Human Lungs
De Man R, Cala Garcia J, Adams T, Nekola F, Moss B, Waich A, Rangel R, Anderson S, Mcdonough J, Raredon M, Yan X, Rosas I, Kaminski N. Single-cell Analysis Reveals Somatic Mutation Accumulation, Loss of Differentiation, and Increased Transcriptional Entropy in Aged Human Lungs. American Journal Of Respiratory And Critical Care Medicine 2025, 211: a5278-a5278. DOI: 10.1164/ajrccm.2025.211.abstracts.a5278.Peer-Reviewed Original ResearchThe Human Proximal Pulmonary Artery Plays a Critical Role in Regulating Cardiopulmonary Function in Health and Disease
Manning E, Lee S, Ashadujjaman M, De Man R, Cai Z, Adams T, Raredon M, Ramachandra A, Cavinato C, Heerdt P, Singh I, Choudhary G, Yan X, Bellini C, Tellides G, Humphrey J, Kaminski N. The Human Proximal Pulmonary Artery Plays a Critical Role in Regulating Cardiopulmonary Function in Health and Disease. American Journal Of Respiratory And Critical Care Medicine 2025, 211: a2891-a2891. DOI: 10.1164/ajrccm.2025.211.abstracts.a2891.Peer-Reviewed Original ResearchEpigenetic age acceleration in idiopathic pulmonary fibrosis revealed by DNA methylation clocks
Kurbanov D, Ahangari F, Adams T, De Man R, Tang J, Carlon M, Abu Hussein N, Cortesi E, Zapata M, De Sadelaar L, Wuyts W, Vanaudenaerde B, Kaminski N, McDonough J. Epigenetic age acceleration in idiopathic pulmonary fibrosis revealed by DNA methylation clocks. American Journal Of Physiology - Lung Cellular And Molecular Physiology 2025, 328: l456-l462. PMID: 39970931, PMCID: PMC12169420, DOI: 10.1152/ajplung.00171.2024.Peer-Reviewed Original ResearchConceptsIdiopathic pulmonary fibrosisIdiopathic pulmonary fibrosis tissuePulmonary fibrosisLung tissueEpigenetic clocksPotential of DNA methylationDNA methylation levelsDebilitating lung diseaseIllumina MethylationEPIC arrayHuman lung tissueEpigenetic ageDNA methylation clocksBiological ageAffected lung tissueIPF casesClinical prognosisMethylation patternsDNA methylationLung diseaseHealthy controlsAcceleration of biological agingMethylation levelsMethylationEPIC arrayAge accelerationClinical assessment
2024
SINGLE-CELL ANALYSIS OF SOMATIC MUTATIONS IN HUMAN LUNG REVEALS ASSOCIATION WITH TRANSCRIPTIONAL CHANGES IN AGING
De Man R, Adams T, McDonough J, Cala-Garcia J, Moss B, Yan X, Rosas I, Kaminski N. SINGLE-CELL ANALYSIS OF SOMATIC MUTATIONS IN HUMAN LUNG REVEALS ASSOCIATION WITH TRANSCRIPTIONAL CHANGES IN AGING. Innovation In Aging 2024, 8: 571-572. PMCID: PMC11690935, DOI: 10.1093/geroni/igae098.1872.Peer-Reviewed Original ResearchSomatic mutationsMutational burdenCell type annotationAlveolar type 1DNA damage response genesAnalysis of somatic mutationsSomatic mutation accumulationAccumulation of somatic mutationsCell typesUbiquitin ligase geneDamage response genesLoss of cell functionAnalyzed somatic mutationsDecreased expressionSingle-cell RNAseqLigase geneMutation accumulationTop genesSignaling GenesCell marker genesResponse genesTranscriptional changesAlveolar type 1 cellsGenesMarker genesPredicting lung aging using scRNA-Seq data
Song Q, Singh A, McDonough J, Adams T, Vos R, De Man R, Myers G, Ceulemans L, Vanaudenaerde B, Wuyts W, Yan X, Schupp J, Hagood J, Kaminski N, Bar-Joseph Z. Predicting lung aging using scRNA-Seq data. PLOS Computational Biology 2024, 20: e1012632. PMID: 39700255, PMCID: PMC11741621, DOI: 10.1371/journal.pcbi.1012632.Peer-Reviewed Original Research
2019
Hybrid Neural Networks for Mortality Prediction from LDCT Images
Yan P, Guo H, Wang G, De Man R, Kalra MK. Hybrid Neural Networks for Mortality Prediction from LDCT Images. Annual International Conference Of The IEEE Engineering In Medicine And Biology Society (EMBC) 2019, 00: 6243-6246. PMID: 31947269, DOI: 10.1109/embc.2019.8857180.Peer-Reviewed Original ResearchConceptsLow-dose CTMortality predictionLung cancer patientsCancer mortality predictionsMortality risk predictionLung cancer subjectsCause mortalityHigh morbidityCancer patientsLung cancerImaging featuresCardiovascular diseaseCancer subjectsHigh riskMortality riskRisk scoreMortality rateClinical practiceDeadly diseaseRisk predictionLDCT imagesDiseaseRiskSame populationScoring methodComparison of deep learning and human observer performance for lesion detection and characterization
De Man R, Gang G, Li X, Wang G. Comparison of deep learning and human observer performance for lesion detection and characterization. Proceedings Of SPIE--the International Society For Optical Engineering 2019, 11072: 110721f-110721f-5. DOI: 10.1117/12.2532331.Peer-Reviewed Original Research