Maura Lane, RVT, RLATG
Research Associate PediatricsDownloadHi-Res Photo
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Titles
Research Associate Pediatrics
Departments & Organizations
- Khokha Lab
Education & Training
- BA
- UC Berkeley, Environmental Science (1988)
Research
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Overview
I am interested in working with and developing new technologies to facilitate our lab's research into congenital malformations. Recently I have used Tol1 and Tol2 transposon technology to create research models with tissue specific fluorescent expression. My current project involves using CRISPR technology to create Knock-in and KO transgenic lines.
CRISPR technology
Transgenic technology
RNA sequencing expression profiling
Medical Research Interests
Animal Diseases; Cardiovascular Abnormalities; Congenital Abnormalities; Disease Models, Animal; Diseases; Infant, Newborn, Diseases; Organisms
Research at a Glance
Yale Co-Authors
Frequent collaborators of Maura Lane's published research.
Publications Timeline
A big-picture view of Maura Lane's research output by year.
Emily Kathryn Mis, PhD
Engin Deniz, MD
11Publications
457Citations
Publications
2021
Microinjection of Xenopus tropicalis Embryos.
Lane M, Mis EK, Khokha MK. Microinjection of Xenopus tropicalis Embryos. Cold Spring Harbor Protocols 2021, 2022: pdb.prot107644. PMID: 34244348, DOI: 10.1101/pdb.prot107644.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsEmbryo sizeLarger embryo sizeTargeted gene manipulationEmbryo development timeSmall embryo sizeXenopus tropicalis embryosDevelopmental biologyEarly embryosMicroinjection protocolGene manipulationGenetic studiesFirst divisionXenopusEmbryosImportant modelDevelopment timeMicroinjectionMorpholinoCRISPRBiologySpeciesDNAMRNAOocytesFertilizationXenopus Tadpole Craniocardiac Imaging Using Optical Coherence Tomography.
Deniz E, Mis EK, Lane M, Khokha MK. Xenopus Tadpole Craniocardiac Imaging Using Optical Coherence Tomography. Cold Spring Harbor Protocols 2021, 2022: pdb.prot105676. PMID: 34031211, PMCID: PMC11995308, DOI: 10.1101/pdb.prot105676.Peer-Reviewed Original ResearchCitationsAltmetricObtaining Xenopus tropicalis Eggs.
Lane M, Mis EK, Khokha MK. Obtaining Xenopus tropicalis Eggs. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106344. PMID: 34031209, DOI: 10.1101/pdb.prot106344.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsConceptsDevelopmental biologyGene manipulation toolsPowerful model systemCell biological studiesCell-free systemTetraploid genomeDiploid genomeThousands of eggsEgg extractsGenetic studiesXenopusGenomePremier systemModel systemEggsBiological studiesBiologyEmbryosFrogsManipulation toolsTiming of stepsSpeciesHormoneFemalesCellsRaising and Maintaining Xenopus tropicalis from Tadpole to Adult
Lane M, Slocum M, Khokha MK. Raising and Maintaining Xenopus tropicalis from Tadpole to Adult. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106369. PMID: 34031210, DOI: 10.1101/pdb.prot106369.Peer-Reviewed Original ResearchCitationsMeSH Keywords and ConceptsObtaining Xenopus tropicalis Embryos by In Vitro Fertilization.
Lane M, Khokha MK. Obtaining Xenopus tropicalis Embryos by In Vitro Fertilization. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106351. PMID: 34031212, DOI: 10.1101/pdb.prot106351.Peer-Reviewed Original ResearchCitationsAltmetricObtaining Xenopus tropicalis Embryos by Natural Mating.
Lane M, Khokha MK. Obtaining Xenopus tropicalis Embryos by Natural Mating. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106609. PMID: 34031213, DOI: 10.1101/pdb.prot106609.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and Concepts
2018
CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis
Deniz E, Mis EK, Lane M, Khokha MK. CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis. Methods In Molecular Biology 2018, 1865: 163-174. PMID: 30151766, DOI: 10.1007/978-1-4939-8784-9_12.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsCardiac developmentCRISPR/Candidate genesHigh-density SNP arrayCRISPR/Cas9 systemGenome editing technologyCongenital heart disease genesNew genomic technologiesHeart disease genesCopy number variationsRapid functional assayXenopus tropicalisCas9 systemGenetic basisDevelopmental systemsEditing technologyGenomic technologiesSequence variationDisease genesDifferent genesGenetic analysisSNP arrayDevelopmental mechanismsMolecular mechanismsWhole-exome sequencing
2017
CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing
Moreno-Mateos MA, Fernandez JP, Rouet R, Vejnar CE, Lane MA, Mis E, Khokha MK, Doudna JA, Giraldez AJ. CRISPR-Cpf1 mediates efficient homology-directed repair and temperature-controlled genome editing. Nature Communications 2017, 8: 2024. PMID: 29222508, PMCID: PMC5722943, DOI: 10.1038/s41467-017-01836-2.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsConceptsHomology-directed repairCpf1 activityGenome editingDifferent eukaryotic systemsGenome engineering toolsEfficient homology-directed repairPost-translational modulationEctothermic organismsEctothermic speciesEukaryotic systemsDNA endonucleaseCRISPR-Cpf1Efficient mutagenesisGenomic DNADNA integrationMolecular understandingTemporal controlZebrafishAsCpf1Cpf1LbCpf1EditingNovel classGenomeMutagenesis
2013
Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis
Lane MA, Kimber M, Khokha MK. Breeding Based Remobilization of Tol2 Transposon in Xenopus tropicalis. PLOS ONE 2013, 8: e76807. PMID: 24116167, PMCID: PMC3792888, DOI: 10.1371/journal.pone.0076807.Peer-Reviewed Original ResearchCitationsAltmetricMeSH Keywords and ConceptsMeSH KeywordsAnimalsAnimals, Genetically ModifiedBase SequenceBinding SitesBreedingCrosses, GeneticDNA Transposable ElementsEmbryo, NonmammalianFemaleGene Expression Regulation, DevelopmentalGreen Fluorescent ProteinsMaleMicroscopy, FluorescenceModels, GeneticMutagenesis, InsertionalReverse Transcriptase Polymerase Chain ReactionTransposasesXenopusConceptsTol2 transposonEnhancer-trapping strategySimple breeding strategiesTransgenic reporter linesX. tropicalisTransgenic linesGene trapBreeding strategiesReporter linesTrap strategyBiological processesTransposonDiverse arrayTransgenesisPowerful modelXenopusGenomeGermlineLinesExpressingRemobilizationTropicalisNew opportunities
2006
Xenopus tropicalis genetics identifies chromatoblast stem cell mutant
Grammer T, Khokha M, Lane M, Harland R. Xenopus tropicalis genetics identifies chromatoblast stem cell mutant. Developmental Biology 2006, 295: 444-445. DOI: 10.1016/j.ydbio.2006.04.369.Peer-Reviewed Original Research
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Pediatrics
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Laboratory for Surgery, Obsterics & Gynecology
375 Congress Avenue, Fl 4, Rm 408
New Haven, CT 06519