Alice Sherrard
Associate Research Scientist in GeneticsCards
About
Research
Publications
2025
ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms
Young L, Sherrard A, Zhou H, Shaikh F, Hutchings J, Riggi M, Narasimhan M, Flaherty W, Bennett E, Rosen M, Giraldez A, Villa E. ExoSloNano: multimodal nanogold labels for identification of macromolecules in live cells and cryo-electron tomograms. Nature Methods 2025, 23: 131-142. PMID: 41315814, PMCID: PMC12791015, DOI: 10.1038/s41592-025-02928-4.Peer-Reviewed Original ResearchCryo-ETIn situ cryo-electron microscopyNanogold probeLiving cellsCryo-electron tomogramsCryo-EMCryo-electron tomographyCryo-EM dataLow copy numberNative cellular environmentCryo-electron microscopyStructure-function relationshipsNuclear proteinsCopy numberNanogold labelingSize scalesIdentification of macromoleculesCryo-electronProtein labelingMolecular networksCellular environmentProteinCorrelative lightCell viabilityElectron microscopyExpanding the Toolkit for Structural Cell Biology with ExoSloNano
Young L, Sherrard A, Zhou H, Shaikh F, Hutchings J, Riggi M, Narasimhan M, Bennett E, Rosen M, Giraldez A, Villa E. Expanding the Toolkit for Structural Cell Biology with ExoSloNano. Structural Dynamics 2025, 12: a31-a31. PMCID: PMC12585620, DOI: 10.1063/4.0000823.Peer-Reviewed Original ResearchCryo-ETIn situ cryo-electron microscopyNanogold probeCryo-EMCryo-electron tomographyLiving cellsCryo-EM dataEfficient protein labelingLow copy numberNative cellular environmentCryo-electron microscopyStructural cell biologyStructure-function relationshipsNuclear proteinsCopy numberSize scalesCryo-electronProtein labelingMolecular networksCell biologyCellular environmentElectron microscopyProteinBiomolecular complexesCell viabilityGenetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo
Boswell C, Hoppe C, Sherrard A, Miao L, Kojima M, Martino P, Zhao N, Stasevich T, Nicoli S, Giraldez A. Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo. Nature Communications 2025, 16: 5503. PMID: 40595661, PMCID: PMC12216260, DOI: 10.1038/s41467-025-61003-w.Peer-Reviewed Original ResearchConceptsFunction in vivoAffinity reagentsPlanar cell polarity protein Vangl2Gene targetingEndogenous protein localizationProtein function in vivoProtein targets in vivoModel organism communitiesKnock-in alleleConventional gene targetingProtein functionSingle chain variable fragmentTranscription factor NanogProtein localizationTargets in vivoZebrafish developmentVariable fragmentAllelesNative behaviorFluorescence visualizationOrganic communityVangl2AffinityGenesZebrafishA Multi-Modal and Generalizable Nanogold Tag for Cryo-Electron Tomography in Intact Cells
Young L, Sherrard A, Shaikh F, Zhou H, Hutchings J, Rosen M, Giraldez A, Villa E. A Multi-Modal and Generalizable Nanogold Tag for Cryo-Electron Tomography in Intact Cells. Structural Dynamics 2025, 12: a166-a166. DOI: 10.1063/4.0000475.Peer-Reviewed Original ResearchCryo-electron tomographyPer-particle basisSpatial resolutionSubtomogram analysisCryo-electronAssociated with heterochromatinHistone variantsStructure-function-relationshipsRibosomal targetMinimal perturbationIntact cellsTagging methodHomologous moleculesHeterochromatinTarget macromoleculesNative environmentGold nanoparticlesSubtomogramPerturbation
2024
A generalized nanogold tagging system for the identification of macromolecules in in situ tomograms
Young L, Sherrard A, Hutchings J, Villa E. A generalized nanogold tagging system for the identification of macromolecules in in situ tomograms. Biophysical Journal 2024, 123: 421a. DOI: 10.1016/j.bpj.2023.11.2561.Peer-Reviewed Original Research
2023
Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin
Pownall M, Miao L, Vejnar C, M'Saad O, Sherrard A, Frederick M, Benitez M, Boswell C, Zaret K, Bewersdorf J, Giraldez A. Chromatin expansion microscopy reveals nanoscale organization of transcription and chromatin. Science 2023, 381: 92-100. PMID: 37410825, PMCID: PMC10372697, DOI: 10.1126/science.ade5308.Peer-Reviewed Original ResearchConceptsZygotic genome activationTranscriptional elongationExpansion microscopyRNA polymerase IIChromatin regulatory factorsEnhancer-promoter contactsGenome activationChromatin organizationNuclear organizationPolymerase IIPol IIFactor NanogTranscription factorsGene expressionRegulatory factorsChromatinNanoscale organizationNanogTranscriptionElongationNucleosomesUniversal processPromoterEmbryosEnhancerCTLA4 depletes T cell endogenous and trogocytosed B7 ligands via cis-endocytosis
Xu X, Dennett P, Zhang J, Sherrard A, Zhao Y, Masubuchi T, Bui J, Chen X, Hui E. CTLA4 depletes T cell endogenous and trogocytosed B7 ligands via cis-endocytosis. Journal Of Experimental Medicine 2023, 220: e20221391. PMID: 37042938, PMCID: PMC10103642, DOI: 10.1084/jem.20221391.Peer-Reviewed Original ResearchConceptsT cellsMajor histocompatibility complexB7 ligandsHuman regulatory T cellsRegulatory T cellsSurface of T cellsCD28 costimulatory signalsAntigen-presenting-cellsAdaptive immune responsesHuman T cellsT cell coreceptorsCostimulatory signalsLigands CD80CTLA4Cell-intrinsicImmune responseCD28Histocompatibility complexTrogocytosisCD28:B7TregsCD80CD86Extrinsic functionsBlockadeVersatile nanogold tagging system applied to the identification of heterochromatin through cryo-FIB-ET
Young L, Sherrard A, Hutchings J, Giraldez A, Villa E. Versatile nanogold tagging system applied to the identification of heterochromatin through cryo-FIB-ET. Biophysical Journal 2023, 122: 316a. DOI: 10.1016/j.bpj.2022.11.1776.Peer-Reviewed Original Research